平台资源号：

保藏编号：SHMCC E00360

中文名称：人肺癌细胞,ChaGo-K-1,SHMCC E00360

拉丁名称：

模式菌株：

其它保藏中心编号：

来源历史：肺，支气管

收藏时间：

参考用途：
动物种类:人
组织来源:肺，支气管
形态:上皮样，贴壁生长
性别:男性，45岁
细胞描述:人肺癌细胞ChaGo-K-1来源于45岁男性的肺支气管，细胞高表达MUC-1粘蛋白mRNA，低表达MUC-2 mRNA，但不表达MUC-3基因。每个批次均通过本库支原体检测，结果为阴性。经本库STR检测无误。STR鉴定结果为：Amelogenin: X,Y；CSF1PO: 10；D13S317: 11；D16S539: 11；D5S818: 12；D7S820: 8,12；T

生物危害程度：

致病对象：

培养温度：人肺癌细胞ChaGo-K-1完全培养液配方（100 ml）：
RPMI 1640 Medium (Invitrogen, 11875-093) 88 ml
FBS (Gibco) 10 ml
Glutamax (Invitrogen, 35050061) 1 ml
Sodium Pyruvate 100 mM Solution (Invitrogen, 11360070) 1 ml
参考传代比例：1：3

需氧类型：气相：空气，95%；二氧化碳，5%。

提供方式：T25培养瓶,干冰冻存管（额外+150元）

保存温度：液氮

人肺癌细胞ChaGo-K-1完全培养液配方（100 ml）：
RPMI 1640 Medium (Invitrogen, 11875-093) 88 ml
FBS (Gibco) 10 ml
Glutamax (Invitrogen, 35050061) 1 ml
Sodium Pyruvate 100 mM Solution (Invitrogen, 11360070) 1 ml
参考传代比例：1：3
参考传代周期：4~6天
参考换液频率：每周2次
冻存液配方：完全培养液95%，DMSO 5%

Lieblich JM, et al. Ectopic and eutopic secretion of chorionic gonadotropin and its subunits in vitro: Comparison of clonal strains from carcinomas of lung and placenta. J. Natl. Cancer Inst. 56: 911-917, 1976. PubMed: 62839 Rabson AS, et al. Production of human chorionic gonadotropin in vitro by a cell line derived from a carcinoma of the lung. J. Natl. Cancer Inst. 50: 669-674, 1973. PubMed: 4708151 Dahiya R, et al. Mucin synthesis and secretion in various human epithelial cancer cell lines that express the MUC-1 mucin gene. Cancer Res. 53: 1437-1443, 1993. PubMed: 8443822 Tashjian AH Jr., et al. Subunits of human chorionic gonadotropin: unbalanced synthesis and secretion by clonal cell strains derived from a bronchogenic carcinoma. Proc. Natl. Acad. Sci. USA 70: 1419-1422, 1973. PubMed: 4514312 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.