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人骨肉瘤细胞,U-2 OS,SHMCC E00347

菌株编号:SHMCC E00347

中文名称:人骨肉瘤细胞,U-2 OS,SHMCC E00347

拉丁名称:

价格:¥2000

状态:咨询客服

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保藏编号:SHMCC E00347

中文名称:人骨肉瘤细胞,U-2 OS,SHMCC E00347

拉丁名称:

模式菌株:

其它保藏中心编号:

来源历史:骨

收藏时间:

参考用途:
动物种类:人
组织来源:骨
形态:上皮样,贴壁生长
性别:女性,15岁
细胞描述:U-2 OS细胞来源于15岁白人女性,U-2 OS细胞与WI-38共培养或CF检测SV40,RSV或腺病毒时未检测到病毒。1972年曾发现被支原体污染并已去除。在我库通过支原体检测为阴性。冻存时为P36代。经本库STR检测结果无误。STR结果如下:Amelogenin: X;CSF1PO: 13;D13S317: 13;D16S539: 11,12;D5S818: 11;D7S820

生物危害程度:

致病对象:

培养温度:人骨肉瘤细胞U-2 OS完全培养液配方(100 ml):
McCoy's 5a Medium (Invitrogen, 12330-031) 90 ml
FBS (Gibco) 10 ml

需氧类型:气相:空气,95%;二氧化碳,5%。

提供方式:T25培养瓶,干冰冻存管(额外+150元)

保存温度:液氮


培养条件

人骨肉瘤细胞U-2 OS完全培养液配方(100 ml):
McCoy's 5a Medium (Invitrogen, 12330-031) 90 ml
FBS (Gibco) 10 ml

参考文献

Heldin CH, et al. A human osteosarcoma cell line secretes a growth factor structurally related to a homodimer of PDGF A-chains. Nature 319: 511-514, 1986. PubMed: 3456080
Ponten J, Saksela E. Two established in vitro cell lines from human mesenchymal tumours. Int. J. Cancer 2: 434-447, 1967. PubMed: 6081590
Raile K, et al. Human osteosarcoma (U-2 OS) cells express both insulin-like growth factor-I (IGF-I) receptors and insulin-like growth factor-II/mannose-6- phosphate (IGF-II/M6P) receptors and synthesize IGF-II: autocrine growth stimulation by IGF-II via the IGF-I receptor. J. Cell. Physiol. 159 : 531-541 , 1994. PubMed: 8188767
Landers JE, et al. Translational enhancement of mdm2 oncogene expression in human tumor cells containing a stabilized wild-type p53 protein. Cancer Res. 57: 3562-3568, 1997. PubMed: 9270029
Moradpour D, et al. Characterization of cell lines allowing titghtly regulated expression of heapatitis C virus core protein. Virology 222: 51-63, 1996. PubMed: 8806487
Ponten J, Saksela E. Two established in vitro cell lines from human mesenchymal tumours. Int. J. Cancer 2: 434-447, 1967. PubMed: 6081590