平台资源号：

保藏编号：SHMCC E00345

中文名称：人肺癌细胞,NCI-H23 [H23],SHMCC E00345

拉丁名称：

模式菌株：

其它保藏中心编号：

来源历史：肺

收藏时间：

参考用途：
动物种类:人
组织来源:肺
形态:上皮样，贴壁
性别:男性，51岁
细胞描述:人非小细胞肺癌细胞，分离自51岁黑人男性肺癌患者治疗前的肺癌组织，携带K-ras 12 突变，突变位于p53基因的246密码子位置， (ATC -> ATG, isoleucine -> methionine). 表达 C-myc, L-myc, v-src, v-abl, v-erb B, c-raf 1, Ha-ras, Ki-ras, N-ras RNAs。每个批次均通过本库支原体

生物危害程度：

致病对象：

培养温度：人肺癌细胞NCI- H23完全培养液配方（100 ml）：

RPMI 1640 Medium (Invitrogen, 11875-093) 88 ml

FBS (Gibco) 10 ml

Glutamax（invitrogen 35050061） 1 ml

Sodium Pyruvate 100 mM Solution (invitrogen 11360070)

需氧类型：气相：空气，95%；二氧化碳，5%。

提供方式：T25培养瓶,干冰冻存管（额外+150元）

保存温度：液氮

人肺癌细胞NCI- H23完全培养液配方（100 ml）：

RPMI 1640 Medium (Invitrogen, 11875-093) 88 ml

FBS (Gibco) 10 ml

Glutamax（invitrogen 35050061） 1 ml

Sodium Pyruvate 100 mM Solution (invitrogen 11360070) 1 ml

Little CD, et al. Amplification and expression of the c-myc oncogene in human lung cancer cell lines. Nature 306: 194-196, 1983. PubMed: 6646201 Takahashi T, et al. p53: A frequent target for genetic abnormalities in lung cancer. Science 246: 491-494, 1989. PubMed: 2554494 Mitsudomi T, et al. p53 gene mutations in non-small-cell lung cancer cell lines and their correlation with the presence of ras mutations and clinical features. Oncogene 7: 171-180, 1992. PubMed: 1311061 Brower M, et al. Growth of cell lines and clinical specimens of human non-small cell lung cancer in a serum-free defined medium. Cancer Res. 46: 798-806, 1986. PubMed: 3940644 Broers JL, et al. Spontaneous changes in intermediate filament protein expression patterns in lung cancer cell lines. J. Cell Sci. 91: 91-108, 1988. PubMed: 2473086 Forsberg K, et al. Expression of functional PDGF beta receptors in a human large-cell lung- carcinoma cell line. Int. J. Cancer 53: 556-560, 1993. PubMed: 8382192 Gazdar AF, et al. Establishment of continuous, clonable cultures of small-cell carcinoma of lung which have amine precursor uptake and decarboxylation cell properties. Cancer Res. 40: 3502-3507, 1980. PubMed: 6108156 NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996. Lung Cancer 4: 155-161, 1988.