平台资源号：

保藏编号：SHMCC E00340

中文名称：人结直肠腺癌细胞,Caco-2 [Caco2],SHMCC E00340

拉丁名称：

模式菌株：

其它保藏中心编号：

来源历史：结肠

收藏时间：

参考用途：
动物种类:人；白人
组织来源:结肠
形态:贴壁生长；细胞形态不均一，有部分含有液泡的巨大细胞
性别:男性，72岁
细胞描述:人结直肠腺癌细胞Caco-2 [Caco2]分离自直肠原位癌，适于转染。当细胞长满时，可表现出肠上皮细胞分化的特征。Caco-2细胞表达维甲酸结合蛋白I和维甲酸结合蛋白II。每个批次均通过本库支原体检测，结果为阴性。冻存时为P31代。经本库STR检测无误，STR鉴定结果为：Amelogenin: X,CSF1PO: 11,D13S317: 1

生物危害程度：

致病对象：

培养温度：人结直肠腺癌细胞Caco-2完全培养液 配方（100 ml）：
MEM (Invitrogen，11090081) 77 ml
FBS (Gibco) 20 ml
Glutamax（invitrogen 35050061） 1 ml
Non-essential Amino Acids, 100X (Invitrogen, 11140050) 1 ml
Sodium Pyruvate 100 mM Solution（invitrogen

需氧类型：气相：空气，95%；二氧化碳，5%。

提供方式：T25培养瓶,干冰冻存管（额外+150元）

保存温度：液氮

人结直肠腺癌细胞Caco-2完全培养液 配方（100 ml）：
MEM (Invitrogen，11090081) 77 ml
FBS (Gibco) 20 ml
Glutamax（invitrogen 35050061） 1 ml
Non-essential Amino Acids, 100X (Invitrogen, 11140050) 1 ml
Sodium Pyruvate 100 mM Solution（invitrogen 11360070） 1 ml
参考传代周期：4-6天
参考传代比例：1 : 3
备注：传代后细胞贴壁较慢，48h内不要换液；细胞形态不均一，有一些含有液泡的巨大细胞，属正常现象；消化时间5-10 min。
参考换液频率：每周2-3次
冻存液：完全培养液95%，DMSO 5%
气相：空气，95%；二氧化碳，5%。 温度：37摄氏度。

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