平台资源号：

保藏编号：SHMCC E00339

中文名称：人前列腺细胞RWPE-1,RWPE-1,SHMCC E00339

拉丁名称：

模式菌株：

其它保藏中心编号：

来源历史：前列腺

收藏时间：

参考用途：
动物种类:人；白人
组织来源:前列腺
形态:上皮样，贴壁生长
性别:男性，54岁
细胞描述:一位54岁白人男性的正常前列腺组织切片的周围区域的上皮细胞用单拷贝的人乳头瘤病毒18（HPV-18）进行转化，建立了RWPE-1。据报道，RWPE-1细胞在三维Matrigel培养时，在雄激素刺激下，形成腺胞（acini）并向培养基中分泌PSA。当与Matrigel或基质细胞混合注射雄性裸鼠时，RWPE-1细胞也能形成腺胞并产生PSA。来源于RWPE-1的细胞用Kirsti

生物危害程度：

致病对象：

培养温度：1. 人前列腺细胞RWPE-1培养液配方：Keratinocyte-SFM (Invitrogen, 17005-042)

K-SFM（Invitrogen，17005-042）培养液包含两个组份：基础培养液1瓶（货号10724-011）+生长因子2管（货号37000-015 ）。按照K-SFM培养液说明书，需额外添加Gentamicin Solution。建议使用Gentamicin/Amphotericin Solution 500X（Gibco, R-015-10）。

需氧类型：气相：空气，95%；二氧化碳，5%。

提供方式：T25培养瓶,干冰冻存管（额外+150元）

保存温度：液氮

1. 人前列腺细胞RWPE-1培养液配方：Keratinocyte-SFM (Invitrogen, 17005-042)

K-SFM（Invitrogen，17005-042）培养液包含两个组份：基础培养液1瓶（货号10724-011）+生长因子2管（货号37000-015 ）。按照K-SFM培养液说明书，需额外添加Gentamicin Solution。建议使用Gentamicin/Amphotericin Solution 500X（Gibco, R-015-10）。

注意事项：

a. 细胞在无血清培养体系中培养，其贴壁能力较弱，建议传代后静置24小时再进行后续操作。

b. 细胞密度建议保持在4万~ 7万cells/cm2，接种密度建议在2万~4万活细胞/cm2。

c. 使用0.05%胰酶消化细胞。同时由于K-sfm为无血清培养液无法终止胰酶消化，所以消化完成后要通过添加2%FBS（用D-PBS稀释）或等体积的0.1%大豆胰蛋白酶抑制剂终止消化并离心去除胰酶，再进行后续操作。

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