菌株编号:SHMCC E00337
中文名称:人非小细胞肺癌细胞,NCI-H1299,SHMCC E00337
拉丁名称:
价格:¥1600
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保藏编号:SHMCC E00337
中文名称:人非小细胞肺癌细胞,NCI-H1299,SHMCC E00337
拉丁名称:
模式菌株:
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来源历史:肺癌;非小细胞肺癌;转移灶;淋巴结
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参考用途:动物种类:人组织来源:肺癌;非小细胞肺癌;转移灶;淋巴结形态:上皮细胞性别:男细胞描述:这株细胞来源于一个淋巴结转移。 患者接受了初期放疗。 细胞均一性的部分缺失p53蛋白,并缺少p53蛋白表达。 细胞可以合成0.1pmol/毫克蛋白的NMB蛋白,而不合成促胃液释放肽(GRP)。 在本库通过支原体检测。 在本库通过STR检测。
生物危害程度:
致病对象:
培养温度:RPMI-1640(GIBCO,货号31800022, 添加NaHCO3 1.5g/L, glucose 2.5g/L, Sodium Pyruvate 0.11g/L),90%;优质胎牛血清,10%。气相:空气,95%;二氧化碳,5%。温度:37摄氏度。
需氧类型:气相:空气,95%;二氧化碳,5%。
提供方式:T25培养瓶,干冰冻存管(额外+150元)
保存温度:液氮
RPMI-1640(GIBCO,货号31800022, 添加NaHCO3 1.5g/L, glucose 2.5g/L, Sodium Pyruvate 0.11g/L),90%;优质胎牛血清,10%。气相:空气,95%;二氧化碳,5%。温度:37摄氏度。
Giaccone G , et al. Neuromedin B is present in lung cancer cell lines. Cancer Res. 52:2732s-2736s, 1992. PubMed: 1563005NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24:1996.Lin DL , Chang C . p53 is a mediator for radiation-repressed human TR2 orphan receptor expression in MCF-7 cells, a new pathway from tumor suppressor to member of the steroid receptor superfamily. J. Biol. Chem. 271: 14649-14652, 1996. PubMed: 8663350 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. Centers for Disease Control (1993), Biosafety in Microbiological and Biomedical Laboratories Human Health Service Publication No. (CDC) 93-8395. U.S. Dept. of Health and Human Services; 3rd Edition U.S. Government Printing Office Washington D.C.