平台资源号：

保藏编号：SHMCC E00311

中文名称：人肝癌细胞,Hep G2 [HEPG2],SHMCC E00311

拉丁名称：

模式菌株：

其它保藏中心编号：

来源历史：肝脏

收藏时间：

参考用途：
动物种类:人
组织来源:肝脏
形态:上皮样，贴壁生长
性别:男性，15岁
细胞描述:人肝癌细胞Hep G2 [HEPG2]来源于一个15岁的白人男性肝癌患者。该细胞表达3-羟基-3-甲基戊二酰还原酶(3-hydroxy-3-methylglutaryl-CoA)和肝三酰甘油脂肪(hepatic triglyceride lipase)活性。此细胞不携带乙肝病毒基因组(Hepatitis B virus)。模式染色体数为55（50-60不等）。在免疫抑制小鼠中不致瘤

生物危害程度：

致病对象：

培养温度：人肝癌细胞 Hep G2完全培养液 配方（100 ml）：
MEM (Invitrogen，11090081) 87 ml
FBS (Gibco) 10 ml
Glutamax（invitrogen 35050061） 1 ml
Non-essential Amino Acids, 100x (Invitrogen, 11140050) 1 ml
Sodium Pyruvate 100 mM Solution（invitrogen 1

需氧类型：气相：空气，95%；二氧化碳，5%。

提供方式：T25培养瓶,干冰冻存管（额外+150元）

保存温度：液氮

人肝癌细胞 Hep G2完全培养液 配方（100 ml）：
MEM (Invitrogen，11090081) 87 ml
FBS (Gibco) 10 ml
Glutamax（invitrogen 35050061） 1 ml
Non-essential Amino Acids, 100x (Invitrogen, 11140050) 1 ml
Sodium Pyruvate 100 mM Solution（invitrogen 11360070） 1 ml
参考传代周期：4-6天
参考传代比例：1:3
参考换液频率：传代时换液
冻存液：完全培养液95%，DMSO 5%
细胞状态：贴壁生长
气相：空气，95%；二氧化碳，5%。温度：37摄氏度。

备注：Hep G2 [HEPG2]对培养条件要求较严格，特别是pH值和血清质量，否则可能影响细胞凝集数量，应使用高质量低内毒素，未灭火的胎牛血清，可以帮助圆形的细胞丛簇更好的贴壁及形成单层细胞。细胞内容易有空泡，特别是在融合时。

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