平台资源号：

保藏编号：SHMCC E00279

中文名称：小鼠胚胎干细胞J1,J1,SHMCC E00279

拉丁名称：

模式菌株：

其它保藏中心编号：

来源历史：小鼠胚胎内细胞团

收藏时间：

参考用途：
动物种类:小鼠，129S4/SvJae品系
组织来源:小鼠胚胎内细胞团
形态:球形克隆，贴壁生长
性别:雄
细胞描述:J1细胞来源于雄性鼠灰色的129S4/SvJae胚胎。该细胞能进入生殖系。每个批次均通过本库支原体检测，结果为阴性。
培养时需使用CF-1 MEF（SCSP-105R）作为饲养层细胞。

生物危害程度：

致病对象：

培养温度：mES完全培养液配方（600 ml）：
DMEM (Invitrogen 12430) 497 ml
ES级FBS (biochrom S4115) 90 ml
Glutamax (Invitrogen 35050) 6 ml
NEAA (Invitrogen 11140) 6 ml
LIF (Millipore ESG1107) 60 μl（终浓度为1000U/ml）
β-Mer

需氧类型：气相：空气，95%；二氧化碳，5%。

提供方式：T25培养瓶,干冰冻存管（额外+150元）

保存温度：液氮

mES完全培养液配方（600 ml）：
DMEM (Invitrogen 12430) 497 ml
ES级FBS (biochrom S4115) 90 ml
Glutamax (Invitrogen 35050) 6 ml
NEAA (Invitrogen 11140) 6 ml
LIF (Millipore ESG1107) 60 μl（终浓度为1000U/ml）
β-Mer (Invitrogen 21985) 1.5 ml
气相：空气，95%；二氧化碳，5%。温度：37摄氏度。

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