平台资源号：

保藏编号：SHMCC E00269

中文名称：仓鼠肾成纤维细胞,BHK-21 [C-13],SHMCC E00269

拉丁名称：

模式菌株：

其它保藏中心编号：

来源历史：正常肾

收藏时间：

参考用途：
动物种类:仓鼠
组织来源:正常肾
形态:成纤维细胞
性别:
细胞描述:1961年3月，I.A. Macpherson和M.G.P. Stoker从1日龄的仓鼠建立了BHK-21 (C-13)的亲本。随后84天连续培养，仅中断8天进行冻存，通过单细胞分离建立了13号克隆。这株细胞可用作转染宿主，用于表达包含选择及扩增标记的DNA(如Factor VIII,见ATCC CRL-8544)的载体。 在本库通过支原体检测。

生物危害程度：

致病对象：

培养温度：MEM培养基(GIBCO,货号41500034，添加NaHCO3 1.5g/L，Sodium Pyruvate 0.11g/L)，90%；优质胎牛血清，10%。

气相：空气，95%；二氧化碳，5%。
温度：37摄氏度。

需氧类型：气相：空气，95%；二氧化碳，5%。

提供方式：T25培养瓶,干冰冻存管（额外+150元）

保存温度：液氮

MEM培养基(GIBCO,货号41500034，添加NaHCO3 1.5g/L，Sodium Pyruvate 0.11g/L)，90%；优质胎牛血清，10%。

气相：空气，95%；二氧化碳，5%。
温度：37摄氏度。

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