菌株编号:SHMCC E00211
中文名称:杂交瘤(抗CD3),OKT 3,SHMCC E00211
拉丁名称:
价格:¥1800
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保藏编号:SHMCC E00211
中文名称:杂交瘤(抗CD3),OKT 3,SHMCC E00211
拉丁名称:
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来源历史:细胞类型:杂交瘤;B淋巴细胞
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参考用途:动物种类:杂交瘤组织来源:细胞类型:杂交瘤;B淋巴细胞形态:淋巴母细胞性别:细胞描述:用人外周血淋巴细胞免疫小鼠。取其脾细胞与P3x63Ag8.U1骨髓瘤细胞融合。
生物危害程度:
致病对象:
培养温度:IMDM培养基(GIBCO,货号12200036),80%;优质胎牛血清,20%。气相:空气,95%;二氧化碳,5%。温度:37摄氏度。
需氧类型:气相:空气,95%;二氧化碳,5%。
提供方式:T25培养瓶,干冰冻存管(额外+150元)
保存温度:液氮
IMDM培养基(GIBCO,货号12200036),80%;优质胎牛血清,20%。气相:空气,95%;二氧化碳,5%。温度:37摄氏度。
Hoffman RA, et al. Simple and rapid measurement of human T lymphocytes and their subclasses in peripheral blood. Proc. Natl. Acad. Sci. USA 77: 4914-4917, 1980. PubMed: 6968909 Kung PC, Goldstein G. Complement-fixing monoclonal antibody to human T cells, and methods of preparing same. US Patent 4,361,549 dated Nov 30 1982 Tanaka Y, et al. Productive and lytic infection of human CD4+ type 1 helper T cells with macrophage-tropic human immunodeficiency virus type 1. J. Virol. 71: 465-470, 1997. PubMed: 8985372 Solomon KR, et al. The association between glycoslphosphatidylinositol-anchored proteins and hetrotrimeric G protein alpha subunits in lymphocytes. Proc. Natl. Acad. Sci. USA 93: 6053-6058, 1996. PubMed: 8650218 Hutchcroft JE, et al. Differential phosphorylation of the T lymphocyte costimulatory receptor CD28. J. Biol. Chem. 271: 13362-13370, 1996. PubMed: 8662792 Hartley D, Corvera S. Formation of c-Cb1-phosphatidylinositol 3-kinase complexes on lymphocyte membranes by a p56lck-independent mechanism. J. Biol. Chem. 271: 21939-21943, 1996. PubMed: 8702998