产品服务

人纤维肉瘤,HT-1080,SHMCC E00174返回上一页

人纤维肉瘤,HT-1080,SHMCC E00174

菌株编号:SHMCC E00174

中文名称:人纤维肉瘤,HT-1080,SHMCC E00174

拉丁名称:

价格:¥1700

状态:咨询客服

下载:

产品介绍

平台资源号:

保藏编号:SHMCC E00174

中文名称:人纤维肉瘤,HT-1080,SHMCC E00174

拉丁名称:

模式菌株:

其它保藏中心编号:

来源历史:结缔组织;纤维肉瘤

收藏时间:

参考用途:
动物种类:人
组织来源:结缔组织;纤维肉瘤
形态:上皮细胞
性别:男
细胞描述:这株细胞含有活化的N-ras癌基因。 在本库通过支原体检测。 在本库通过STR检测。

生物危害程度:

致病对象:

培养温度:MEM培养基(GIBCO,货号41500034,添加NaHCO3 1.5g/L,Sodium Pyruvate 0.11g/L),90%;优质胎牛血清,10%。

气相:空气,95%;二氧化碳,5%。
温度:37摄氏度。

需氧类型:气相:空气,95%;二氧化碳,5%。

提供方式:T25培养瓶,干冰冻存管(额外+150元)

保存温度:液氮


培养条件

MEM培养基(GIBCO,货号41500034,添加NaHCO3 1.5g/L,Sodium Pyruvate 0.11g/L),90%;优质胎牛血清,10%。

气相:空气,95%;二氧化碳,5%。
温度:37摄氏度。

参考文献

22147: Chen TR , et al. Intercellular karyotypic similarity in near-diploid cell lines of human tumor origins. Cancer Genet. Cytogenet. 10: 351-362, 1983. PubMed: 6652615 23071: Geiser AG , et al. Suppression of tumorigenicity in human cell hybrids derived from cell lines expressing different activated ras oncogenes. Cancer Res. 49: 1572-1577, 1989. PubMed: 2647289 23393: Rasheed S , et al. Characterization of a newly derived human sarcoma cell line (HT-1080). Cancer 33: 1027-1033, 1974. PubMed: 4132053 25969: Adams RA , et al. Direct implantation and serial transplantation of human acute lymphoblastic leukemia in hamsters, SB-2. Cancer Res. 28: 1121-1125, 1968. PubMed: 4872716 26035: Proc. Am. Assoc. Cancer Res. 8: 1, 1967. 32289: Hu M , et al. Purification and characterization of human lung fibroblast motility-stimulating factor for human soft tissue sarcoma cells: identification as an NH2-terminal fragment of human fibronectin. Cancer Res. 57: 3577-3584, 1997. PubMed: 9270031 32370: Iida A , et al. Inducible gene expression by retrovirus-mediated transfer of a modified tetracycline-regulated system. J. Virol. 70: 6054-6059, 1996. PubMed: 8709228 32531: Brenneman M , et al. Stimulation of intrachromosomal homologous recombination in human cells by electroporation with site-specific endonucleases. Proc. Natl. Acad. Sci. USA 93: 3608-3612, 1996. PubMed: 8622983 33061: Seiffert D . Hydrolysis of platelet vitronectin by calpain. J. Biol. Chem. 271: 11170-11176, 1996. PubMed: 8626663 33152: Hocking AM , et al. Eukaryotic expression of recombinant biglycan. J. Biol. Chem. 271: 19571-19577, 1996. PubMed: 8702651