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人肾母细胞瘤细胞,SK-NEP-1,SHMCC E00172

菌株编号:SHMCC E00172

中文名称:人肾母细胞瘤细胞,SK-NEP-1,SHMCC E00172

拉丁名称:

价格:¥2000

状态:咨询客服

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平台资源号:

保藏编号:SHMCC E00172

中文名称:人肾母细胞瘤细胞,SK-NEP-1,SHMCC E00172

拉丁名称:

模式菌株:

其它保藏中心编号:

来源历史:肾,肋膜渗出液

收藏时间:

参考用途:
动物种类:人
组织来源:肾,肋膜渗出液
形态:圆形悬浮
性别:女
细胞描述:超微结构有少许微绒毛,连接复合物,形态完整的高尔基体,内质网多为光滑型,脂滴,没有病毒棵粒。 在本库通过支原体检测。 在本库通过STR检测。

生物危害程度:

致病对象:

培养温度:McCOY's 5A(SIGMA,货号M4892,添加NaHCO3 2.2g/L),85%;优质胎牛血清,15%。

气相:空气,95%;二氧化碳,5%。
温度:37摄氏度。

需氧类型:气相:空气,95%;二氧化碳,5%。

提供方式:T25培养瓶,干冰冻存管(额外+150元)

保存温度:液氮


培养条件

McCOY's 5A(SIGMA,货号M4892,添加NaHCO3 2.2g/L),85%;优质胎牛血清,15%。

气相:空气,95%;二氧化碳,5%。
温度:37摄氏度。

参考文献

Fogh J, editor. Human tumor cells in vitro. New York: Plenum Press; 1975, pp. 115-159.
Fogh J , et al. Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J. Natl. Cancer Inst. 58: 209-214, 1977. PubMed: 833871
Fogh J , et al. One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J. Natl. Cancer Inst. 59: 221-226, 1977. PubMed: 327080
Fogh J . Cultivation, characterization, and identification of human tumor cells with emphasis on kidney, testis, and bladder tumors. Natl. Cancer Inst. Monogr. 49: 5-9, 1978. PubMed: 571047
Orkin SH , et al. Development of homozygosity for chromosome 11p markers in Wilms' tumour. Nature 309: 172-174, 1984. PubMed: 6325937
Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.
Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.
Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.
Centers for Disease Control (1993), Biosafety in Microbiological and Biomedical Laboratories Human Health Service Publication No. (CDC) 93-8395. U.S. Dept. of Health and Human Services; 3rd Edition U.S. Government Printing Office Washington D.C.