平台资源号：

保藏编号：SHMCC E00153

中文名称：狗肾细胞,MDCK(NBL-2),SHMCC E00153

拉丁名称：

模式菌株：

其它保藏中心编号：

来源历史：肾

收藏时间：

参考用途：
动物种类:狗
组织来源:肾
形态:上皮细胞
性别:雌
细胞描述:1958年九月 S.H. Madin and N.B. Darby从一只外观正常的成年雌性英国小猎犬的肾分离等到MDCK细胞株。角蛋白免疫过氧化物酶染色呈阳性。MDCK被用于研究β-粥样蛋白前体的处理及其蛋白水解产物。 在本库通过支原体检测。

生物危害程度：

致病对象：

培养温度：MEM培养基(GIBCO,货号41500034，添加NaHCO3 1.5g/L，Sodium Pyruvate 0.11g/L)，90%；优质胎牛血清，10%。

气相：空气，95%；二氧化碳，5%。
温度：37摄氏度。

需氧类型：气相：空气，95%；二氧化碳，5%。

提供方式：T25培养瓶,干冰冻存管（额外+150元）

保存温度：液氮

MEM培养基(GIBCO,货号41500034，添加NaHCO3 1.5g/L，Sodium Pyruvate 0.11g/L)，90%；优质胎牛血清，10%。

气相：空气，95%；二氧化碳，5%。
温度：37摄氏度。

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