平台资源号：

保藏编号：SHMCC E00149

中文名称：小鼠肾小球系膜细胞,SV40 MES 13,SHMCC E00149

拉丁名称：

模式菌株：

其它保藏中心编号：

来源历史：肾小球系膜细胞

收藏时间：

参考用途：
动物种类:小鼠
组织来源:肾小球系膜细胞
形态:心肌成纤维细胞样
性别:
细胞描述:这株细胞的细胞骨架染色突出呈现肌动蛋白，在细胞质中有丰富的平行微丝。 有报道称在1uM血管紧张素II存在下，它们会收缩。 细胞在软琼脂中形成克隆，SV40大T抗原阳性。 在本库通过支原体检测。

生物危害程度：

致病对象：

培养温度：DMEM培养基(GIBCO,货号12800017，添加NaHCO3 1.5g/L)，71.25%；F-12培养基(GIBCO,货号21700075，添加L-glutamine 150mg/L, NaHCO3 1.5g/L)，23.75%；添加14mM HEPES；优质胎牛血清，5%-10%。

气相：空气，95%；二氧化碳，5%。
温度：37摄氏度。

需氧类型：气相：空气，95%；二氧化碳，5%。

提供方式：T25培养瓶,干冰冻存管（额外+150元）

保存温度：液氮

DMEM培养基(GIBCO,货号12800017，添加NaHCO3 1.5g/L)，71.25%；F-12培养基(GIBCO,货号21700075，添加L-glutamine 150mg/L, NaHCO3 1.5g/L)，23.75%；添加14mM HEPES；优质胎牛血清，5%-10%。

气相：空气，95%；二氧化碳，5%。
温度：37摄氏度。

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