平台资源号：

保藏编号：SHMCC E00148

中文名称：大鼠肺泡巨噬细胞,NR8383,SHMCC E00148

拉丁名称：

模式菌株：

其它保藏中心编号：

来源历史：肺；巨噬细胞；肺泡

收藏时间：

参考用途：
动物种类:大鼠
组织来源:肺；巨噬细胞；肺泡
形态:巨噬细胞，半悬浮或悬浮生长并部分聚团
性别:
细胞描述:NR8383 (正常大鼠，1983年8月3日)来源于肺灌洗时的正常大鼠肺泡巨噬细胞。 细胞在gerbil肺细胞连续培养液存在下培养了大约8-9个月。 随后，不再需要外源生长因子。
通过有限稀释法从单个细胞克隆并亚克隆NR8383细胞，并三次用软琼脂亚克隆。 细胞表现出巨噬细胞的特性，吞噬酵母多糖和铜绿，非特异性脂酶活性，Fc受体，氧化降解；分泌IL-

生物危害程度：

致病对象：

培养温度：F12K培养基(SIGMA,货号N3520，添加NaHCO3 2.5g/L)，80%；优质胎牛血清，20%。

气相：空气，95%；二氧化碳，5%。
温度：37摄氏度。

需氧类型：气相：空气，95%；二氧化碳，5%。

提供方式：T25培养瓶,干冰冻存管（额外+150元）

保存温度：液氮

F12K培养基(SIGMA,货号N3520，添加NaHCO3 2.5g/L)，80%；优质胎牛血清，20%。

气相：空气，95%；二氧化碳，5%。
温度：37摄氏度。

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