平台资源号：

保藏编号：SHMCC E00138

中文名称：人急性淋巴细胞白血病T淋巴细胞,CCRF-CEM [CCRF CEM],SHMCC E00138

拉丁名称：

模式菌株：

其它保藏中心编号：

来源历史：白血病, 急性淋巴细胞白血病, 外周血, T淋巴母细胞

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参考用途：
动物种类:人
组织来源:白血病, 急性淋巴细胞白血病, 外周血, T淋巴母细胞
形态:淋巴母细胞
性别:女
细胞描述:G.E. Foley 等人建立了类淋巴母细胞细胞株CCRF-CEM。 细胞是1964年11月从一位四岁白人女性急性淋巴细胞白血病患者的外周血白血球衣中得到。 在本库通过支原体检测。 在本库通过STR检测。

生物危害程度：

致病对象：

培养温度：RPMI-1640(GIBCO,货号31800022, 添加NaHCO3 1.5g/L, glucose 2.5g/L, Sodium Pyruvate 0.11g/L)，90%；优质胎牛血清，10%。

气相：空气，95%；二氧化碳，5%。
温度：37摄氏度。

需氧类型：气相：空气，95%；二氧化碳，5%。

提供方式：T25培养瓶,干冰冻存管（额外+150元）

保存温度：液氮

RPMI-1640(GIBCO,货号31800022, 添加NaHCO3 1.5g/L, glucose 2.5g/L, Sodium Pyruvate 0.11g/L)，90%；优质胎牛血清，10%。

气相：空气，95%；二氧化碳，5%。
温度：37摄氏度。

Foley GE et al. Continous culture of human lymphoblasts from peripheral blood of a child with acute leukemia. Cancer 18: 522-529, 1965
Sandstrom PA and Buttke TM. Autocrine production of extracellular catalase prevents apoptosis of the human CEM T-cell line in serum-free medium. Proc. Natl Acad. Sci. USA 90:4708-4712, 1993 PubMed: 93281648
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Adams RA. Formal discussion: the role of transplantation in the experimental investigation of human leukemia and lymphoma. Cancer Res. 27: 2479-2482, 1967 PubMed: 68134846
Uzman BG et ah Morphologic variations in human leukemic lymphoblasts (CCRF-CEM cells) after long-term culture and exposure to chemotherapeutic agents. A study with the electron microscope. Cancer 19:1725-I742, 1966 PubMed: 67(146473
Adams RA et al. Leukemia: serial transplantation of human leukemic lymphoblasts in the newborn Syrian halnster. Cancer Res. 27: 772-783. 1967 PubMed: 68122005
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Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1 q88.
Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D.,(1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.
Centers for Disease Control (1993), Biosafety in Microbiological and Biomedical Laboratories Human Health Service Publication No. (CDC) 93-8395. U.S Dept. of Health and Human Services; 3rd Edition U.S. Government Printing Office Washington D.C.