菌株编号:SHMCC E00107
中文名称:人结直肠腺癌上皮细胞,DLD-1,SHMCC E00107
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价格:¥1700
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保藏编号:SHMCC E00107
中文名称:人结直肠腺癌上皮细胞,DLD-1,SHMCC E00107
拉丁名称:
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来源历史:结直肠腺癌上皮细胞
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参考用途:动物种类:人组织来源:结直肠腺癌上皮细胞形态:上皮细胞性别:细胞描述:DLD-1 是1977-1979年间D.L. Dexter和同事分离的两株结直肠腺癌细胞株中的一株。 在ATCC和其它地方进行的DNA fingerprinting和染色体组型分析表明这株细胞与HCT-15 (CCL-225)相似,说明这两者是来自同一个人的不同克隆。 他们的遗传起源可通过DNA fingerprinting证实,但染色体组型分析显示它们缺乏染色体标记一致改变或数目上一致
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致病对象:
培养温度:RPMI-1640(GIBCO,货号31800022, 添加NaHCO3 1.5g/L, glucose 2.5g/L, Sodium Pyruvate 0.11g/L),90%;优质胎牛血清,10%。气相:空气,95%;二氧化碳,5%。温度:37摄氏度。
需氧类型:气相:空气,95%;二氧化碳,5%。
提供方式:T25培养瓶,干冰冻存管(额外+150元)
保存温度:液氮
RPMI-1640(GIBCO,货号31800022, 添加NaHCO3 1.5g/L, glucose 2.5g/L, Sodium Pyruvate 0.11g/L),90%;优质胎牛血清,10%。气相:空气,95%;二氧化碳,5%。温度:37摄氏度。
Chen TR , et al. Intercellular karyotypic similarity in near-diploid cell lines of human tumor origins. Cancer Genet. Cytogenet. 10: 351-362, 1983. PubMed: 6652615 22417: Chen TR , et al. DLD-1 and HCT-15 cell lines derived separately from colorectal carcinomas have totally different chromosome changes but the same genetic origin. Cancer Genet. Cytogenet. 81: 103-108, 1995. PubMed: 7621404 22861: Trainer DL , et al. Biological characterization and oncogene expression in human colorectal carcinoma cell lines. Int. J. Cancer 41: 287-296, 1988. PubMed: 3338874 23033: Dexter DL , et al. N,N-dimethylformamide-induced alteration of cell culture characteristics and loss of tumorigenicity in cultured human colon carcinoma cells. Cancer Res. 39: 1020-1025, 1979. PubMed: 427742 23322: Rodrigues NR , et al. p53 mutations in colorectal cancer. Proc. Natl. Acad. Sci. USA 87: 7555-7559, 1990. PubMed: 1699228 23341: Keesee SK , et al. Nuclear matrix proteins in human colon cancer. Proc. Natl. Acad. Sci. USA 91: 1913-1916, 1994. PubMed: 8127905 32794: Kutchera W , et al. Protaglandin H synthase 2 is expressed abnormally in human colon cancer: evidence for a transcriptional effect. Proc. Natl. Acad. Sci. USA 93: 4816-4820, 1996. PubMed: 8643486 46971: Vermeulen SJ , et al. Did the four human cancer cell lines DLD-1, HCT-15, HCT-8, and HRT-18 originate from one and the same patient?. Cancer Genet. Cytogenet. 107: 76-79, 1998. PubMed: 9809040