平台资源号：

保藏编号：SHMCC E00005

中文名称：小鼠胚胎成纤维细胞,NIH/3T3,SHMCC E00005

拉丁名称：

模式菌株：

其它保藏中心编号：

来源历史：胚胎

收藏时间：

参考用途：
动物种类:小鼠
组织来源:胚胎
形态:成纤维细胞
性别:
细胞描述:与原始的随机交配3T3和近交系BALB/c 3T3建株方法一样，NIH/3T3，是从NIH Swiss小鼠胚胎培养物中建立的高度接触抑制的连续细胞株。 为了培育在形态学特征上更适合于进行转化分析的亚株，建立的NIH/3T3细胞株又进行了五轮以上亚克隆。 这株细胞对DNA转化及转染研究十分有用。 检测表明肢骨发育畸形病毒(鼠痘)阴性。
在本库通过支原体检测。

生物危害程度：

致病对象：

培养温度：DMEM培养基(GIBCO,货号12800017，添加NaHCO3 1.5g/L)，90%；优质胎牛血清，10%。 气相：空气，95%；二氧化碳，5%。 温度：37摄氏度。

需氧类型：气相：空气，95%；二氧化碳，5%。

提供方式：T25培养瓶,干冰冻存管（额外+150元）

保存温度：液氮

DMEM培养基(GIBCO,货号12800017，添加NaHCO3 1.5g/L)，90%；优质胎牛血清，10%。 气相：空气，95%；二氧化碳，5%。 温度：37摄氏度。

Jainchill JL , et al. Murine sarcoma and leukemia viruses: assay using clonal lines of contact-inhibited mouse cells. J. Virol. 4: 549-553, 1969. PubMed: 4311790 Andersson P , et al. A defined subgenomic fragment of in vitro synthesized Moloney sarcoma virus DNA can induce cell transformation upon transfection. Cell 16: 63-75, 1979. PubMed: 84715 Copeland NG , Cooper GM . Transfection by exogenous and endogenous murine retrovirus DNAs. Cell 16: 347-356, 1979. PubMed: 222457 Loffler S , et al. CD9, a tetraspan transmembrane protein, renders cells susceptible to canine distemper virus. J. Virol. 71: 42-49, 1997. PubMed: 8985321 Berson JF , et al. A seven-transmembrane domain receptor involved in fusion and entry of T-cell-tropic human immunodeficiency virus tyep 1 strains. J. Virol. 70: 6288-6295, 1996. PubMed: 8709256 Jones PL , et al. Tumor necrosis factor alpha and interleukin-1beta regulate the murine manganese superoxide dismutase gene through a complex intronic enhancer involving C/EBP-beta and NF-kappaB. Mol. Cell. Biol. 17: 6970-6981, 1997. PubMed: 9372929 Gonzalez Armas JC , et al. DNA immunization confers protection against murine cytomegalovirus infection. J. Virol. 70: 7921-7928, 1996. PubMed: 8892915 Siess DC , et al. Exceptional fusogenicity of chinese hamster ovary cells with murine retrovirus suggests roles for cellular factor(s) and receptor clusters in the membrane fusion process. J. Virol. 70: 3432-439, 1996. PubMed: 8648675 Jang SI , et al. Activator protein 1 activity is involved in the regulation of the cell type-specific expression from the proximal promoter of the human profilaggrin gene. J. Biol. Chem. 271: 24105-24114, 1996. PubMed: 8798649 Medin JA , et al. Correction in trans for Fabry disease: expression, secretion, and uptake of alpha-galactosidase A in patient-derived cells driven by a high-titer recombinant retroviral vector. Proc. Natl. Acad. Sci. USA 93: 7917-7922, 1996. PubMed: 8755577 Lee JH , et al. The proximal promoter of the human transglutaminase 3 gene. J. Biol. Chem. 271: 4561-4568, 1996. PubMed: 8626812 Chang K , Pastan I . Molecular cloning of mesothelin, a differentiation antigen present on mesothelium, mesotheliomas, and ovarian cancers. Proc. Natl. Acad. Sci. USA 93: 136-140, 1996. PubMed: 8552591 Cranmer LD , et al. Identification, analysis, and evolutionary relationships of the putative murine cytomegalovirus homologs of the human cytomegalovirus UL82 (pp71) and UL83 (pp65) matrix phosphoproteins. J. Virol. 70: 7929-7939, 1996. PubMed: 8892916 Shisler J , et al. Induction of susceptibility to tumor necrosis factor by E1A is dependent on binding to either p300 or p105-Rb and induction of DNA synthesis. J. Virol. 70: 68-77, 1996. PubMed: 8523594 Cavanaugh VJ , et al. Murine cytomegalovirus with a deletion of genes spanning HindIII-J and -I displays altered cell and tissue tropism. J. Virol. 70: 1365-1374, 1996. PubMed: 8627652 Westerman KA , Leboulch P . Reversible immortalization of mammalian cells mediated by retroviral transfer and site-specific recombination. Proc. Natl. Acad. Sci. USA 93: 8971-8976, 1996. PubMed: 8799138 Tumor 22(1):5-8,2002 Chin J Cell Mol Immunol 18(2):146-148, 2002 Chin J Immunol 18(1):33-35,2002 Acta Biologiae Experimentalis Sinica 36(1):5-12,2003 J Fujian Med Univ 37(2):144-146,2003 Shanghai Immunol Journal 23(1):16-19,2003