平台资源号：

保藏编号：SHMCC E00004

中文名称：小鼠胚胎成纤维细胞,BALB/3T3 clone A31,SHMCC E00004

拉丁名称：

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来源历史：器官：胚胎 品系：BALB/c 细胞类型：成纤维细胞

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参考用途：
动物种类:小鼠
组织来源:器官：胚胎 品系：BALB/c 细胞类型：成纤维细胞
形态:成纤维细胞
性别:
细胞描述:BALB/3T3 clone A31 是 1968年S.A. Aaronson和 G.T. Todaro从裂解的14到17天的 BALB/c 小鼠胚胎中建立的几株细胞之一(参见ATCC CCL-164,BALB/3T12)。 细胞分裂对接触抑制特别敏感，生长需高倍数稀释，饱和浓度低，对癌基因DNA病毒SV40和小鼠肉瘤病毒高度易感。 检测发现鼠痘病毒

生物危害程度：

致病对象：

培养温度：DMEM培养基(GIBCO,货号12800017，添加NaHCO3 1.5g/L)，90%；优质胎牛血清，10%。 气相：空气，95%；二氧化碳，5%。 温度：37摄氏度。

需氧类型：气相：空气，95%；二氧化碳，5%。

提供方式：T25培养瓶,干冰冻存管（额外+150元）

保存温度：液氮

DMEM培养基(GIBCO,货号12800017，添加NaHCO3 1.5g/L)，90%；优质胎牛血清，10%。 气相：空气，95%；二氧化碳，5%。 温度：37摄氏度。

22708: Aaronson SA, Todaro GJ. Development of 3T3-like lines from Balb-c mouse embryo cultures: transformation susceptibility to SV40. J. Cell. Physiol. 72: 141-148, 1968. PubMed: 4301006 26022: Todaro GJ, Aaronson SA. Properties of clonal lines of murine sarcoma virus transformed Balb-3T3 cells. Virology 38: 174-202, 1969. PubMed: 4306523 26023: Aaronson SA, Todaro GJ. Basis for the acquisition of malignant potential by mouse cells cultivated in vitro. Science 162: 1024-1026, 1968. PubMed: 4301647 26024: Jainchill JL, Todaro GJ. Stimulation of cell growth in vitro by serum with and without growth factor. Relation to contact inhibition and viral transformation. Exp. Cell Res. 59: 137-146, 1970. PubMed: 4194429 32554: Thompson SA, et al. COOH-terminal extended recombinant amphiregulin with bioactivity comparable with naturally derived growth factor. J. Biol. Chem. 271: 17927-17931, 1996. PubMed: 8663535 32907: Anderson MT, et al. Simultaneous fluorescence-activated cell sorter analysis of two distinct transcriptional elements within a single cell using engineered green fluorescent proteins. Proc. Natl. Acad. Sci. USA 93: 8508-8511, 1996. PubMed: 8710900 92346: Biological evaluation of medical devices. Part 5: Tests for in vitro cytotoxicity. Sydney, NSW, Australia:Standards Australia;Standards Australia AS ISO 10993.5-2002. 92389: Biological evaluation of medical devices--Part 5: Tests for in vitro cytotoxicity. Geneva (Switzerland):International Organization for Standardization/ANSI;ISO ISO 10993-5:1999.